ATTENUATION OF CROWN GALL BACTERIA BY CULTIVATION IN MEDIA CONTAINING GLYCINE

Respiratory Behavior of Bacteria-Free Crown-Gall Tissues

The respiratory processes of malignant tissues of animals and human beings have long been considered to differ in important respects from those of nonmalignant tissues of the same creatures. This difference expresses itself in several ways, especially in a low R.Q. (0.8 or less) and a decided anaerobic tendency to accumulate acid products, lactic acid in particular. It seems possible, indeed, i...

Tumor Formation by Attenuated Crown-Gall Bacteria in the Presence of Growth-Promoting Substances.

Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced Nitrogen.

Several compounds containing reduced nitrogen markedly increased the yields of cell-division compounds extractable from an A6 Vinca rosea L. crown gall tumor tissue. Casein hydrolysate, several amino acids, and ammonium salts were effective. Both trans-zeatin and ribosyl-trans-zeatin were substantially increased in total amount per culture and in concentration. These two compounds have been ide...

Diffusate media for cultivation of oral anaerobic bacteria.

Antigen-free media suitable for cultivating anaerobic bacteria from the human gingival crevice were devised, based upon supplementing diffusates from standard media ingredients. Growth in these is at least as good as in customary media. Formulations suitable for the major species encountered are described and compared. 概要:口 腔内嫌気性培養用に考案 した透析培地について,真 下 彰*,S・A・ エ リソン*.口 腔内諸 疾患 の免疫学的研究 を,と くに 口内細菌 ...

Evidence for the Presence of Bacteria-specific Proteins in Sterile Crown Gall Tumor Tissue.

Cross-reacting antigens were found in bacteria-free crown gall tumor tissue tested with serum prepared against Agrobacterium tumefaciens (Smith and Towns.) Conn., but no such antigens were detected in callus tissue. Soluble proteins from tumor tissue, callus tissue, and the crown gall bacteria were fractionated on a DEAE-Sephadex (A-50) column. The diethylaminoethyl-Sephadex elution profile for...